Debashish Das, PhD
Principal Investigator drdebashish@narayananethralaya.com+91 80 66660722 (Off), +91 9591889213 (Mob)
Curriculum Vitae
Differential F-actin staining pattern of HCE-T and ARPE-19 cells cultured on Polycaprolactone fibers of 500nm and 1300nm diameters.(Krishna et al., ACS Applied Biomaterials, 2020)
Phagocytosis of FITC-Latex beads by primary Human RPE cells in the presence/absence of bevacizumab and resveratrol.(Subramani et al., Scientific Reports, 2017)
Resveratrol restores Vitamin D receptor levels in HCE-T cells cultured in hyperosmolar conditions. (Shetty et al., Cornea, 2020)
Percentage of corneal stem cells stained by ABCG2 in cultured limbal, conjunctival and buccal epithelial cells.(Dhamodaran et al., Cytotherapy. 2016)
Increase in pro-apoptotic marker (BAX) and decrease in anti-apoptotic marker (BCl2) in the affected cone with increasing grades of keratoconus.(Shetty et al., Trans Vis Sci Tech. 2019)
Research Interests
Investigate resveratrol as a non-invasive therapeutic option in mitigating Keratoconus
Corneal epithelial cells/ fibroblast/ keratocytes
Induce oxidative stress
(Develop keratoconus model ( marker validation))
+/- Resveratrol
Read-out assays
Understanding the molecular mechanism of LncRNA in ocular in health and disease
Tear sample analysis for lncRNA from healthy and diseased patients
Identify the lncRNA in tears samples
Develop invitro disease models- to validate the lncRNA
Identify the interacting partners of lncRNA
Validate lncRNA as diagnostic, prognostic or therapeutic targets
Investigate role of resveratrol on corneal stromal stem cells to differentiate to corneal keratocytes
Establish primary corneal stromal fibroblast cultures
Differentiate to corneal keratocytes
Marker based validation
Explore clinical applicability in corneal stromal keratocyte disease
Select Publications
- Zia Sultan Pradhan, Shivani Dixit, Lekshmi Krishna, Reshma Shetty, Sushma Tejwani, Harsha L Rao, Rohit Shetty, Carroll A B Webers, Debashish Das. Upregulated NOTCH signaling in the lens of patients with pseudoexfoliation syndrome compared to pseudoexfoliation glaucoma suggests protective role. Journal of Glaucoma. 2022; 31(3): e1- e9.
- Rohit Shetty, Nimisha Rajiv Kumar, Murali Subramani, Lekshmi Krishna, Ponnalagu Murugeswari, Himanshu Matalia, Pooja Khamar, Zelda V Dadachanji, Rajiv R Mohan, Arkasubhra Ghosh, Debashish Das. Safety and efficacy of combination of suberoylamilide hydroxyamic acid and mitomycin C in reducing pro-fibrotic changes in human corneal epithelial cells. Scientific Reports. 2021;11(1):4392
- Rohit Shetty, Murali Subramani, Ponnalagu Murugeswari, Venkata Ramana Anandula, Himanshu Matalia, Chaitra Jayadev, Arkasubhra Ghosh, Debashish Das. Resveratrol Rescues Human Corneal Epithelial Cells Cultured in Hyperosmolar Conditions: Potential for Dry Eye Disease Treatment. Cornea. 2020;39(12):1520-1532
- Ponnalagu Murugeswari, Arman Firoz, Subramani Murali, Anand Vinekar, Lekshmi Krishna, Venkata Ramana Anandula, Nallathambi Jeyabalan, Priyanka Chevour, Chaitra Jayadev, Rohit Shetty, Gilles Carpentier, Govindaswamy Kumaramanickavel, Arkasubhra Ghosh, Debashish Das. Vitamin-D3 (α-1, 25(OH) 2D3) protects retinal pigment epithelium from hyperoxic insults. Investigative Ophthalmology & Visual Science. Feb, 2020, 61(2):4
- Lekshmi Krishna, Sagar Nilawar, Murugeswari Ponnalagu, Murali Subramani, Chaitra Jayadev, Rohit Shetty, Kaushik Chatterjee, Debashish Das. Fiber Diameter Differentially Regulates Function of Retinal Pigment and Corneal Epithelial Cells on Nanofibrous Tissue Scaffolds. ACS Applied Biomaterials. Jan, 2020, 3, 2, 823–837
- Rohit Shetty , Krishna P. Vunnava , Kamesh Dhamodaran , Himanshu Matalia , Subramani Murali , Chaitra Jayadev , Ponnalagu Murugeswari , Arkasubhra Ghosh, Debashish Das. Characterization of corneal epithelial cells in keratoconus. Translational Vision Science and Technology, Jan 2018, (8), 2
Short CV
EDUCATION:
2001-2005- PhD- Max Delbrueck Center for Molecular Medicine and Freie University, GERMANY 1999-2000-M.Phil-University of Madras, INDIA 1995-1998-M.S.-Birla Institute of Technology and Sankara Netralaya, INDIA
PROFESSIONAL APPOINTMENTS:
Nov, 2010- Present: Chief Scientist Basic Science R&D April, 2009- Oct, 2010: Senior Scientist April, 2010- Oct, 2010: Visiting Researcher March, 2006- Dec, 2008: Postdoctoral Fellow Oct 1999- Dec, 2000: Research Fellow
RESEARCH GRANTS:
Department of Science & Technology, Government of India National Keratoconus Foundation, USA Department of Science & Technology, Biocare, Government of Velux Stiftung, Switzerland
PUBLICATIONS:
CONFERENCES ORGANIZED:
2 (Regenerative and Transplantation Medicine in Ophthalmology, 12-16 Nov, 2011, Bangalore; Co-Organizer of International Society for Genetic Eye Diseases and Retinoblastoma, 14-16 Jan, 2011, Bangalore, India)